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KMID : 1161520230270010083
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Animal Cells and Systems
2023 Volume.27 No. 1 p.83 ~ p.93
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Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium
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Kim Ji-Eun
Kim Eun-Mi
Lee Hyang-Ae
Kim Ki-Suk
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Abstract
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Cardiomyocytes derived from human pluripotent stem cells (hPSCs) can be used in various applications including disease modeling, drug safety screening, and novel cell-based cardiac therapies. Here, we report an optimized selection and maturation method to induce maturation of cardiomyocytes into a specific subtype after differentiation driven by the regulation of Wnt signaling. The medium used to optimize selection and maturation was in a glucose starvation conditions, supplemented with either a nutrition complex or ascorbic acid. Following optimized selection and maturation, more cardiac Troponin T (cTnT)-positive cardiomyocytes were detected using albumin and ascorbic acid than B27. In addition, ascorbic acid enriched maturation of ventricular cardiomyocytes. We compared cardiomyocyte-specific gene expression patterns under different selection and maturation conditions by next-generation sequencing (NGS) analysis. Our optimized conditions will enable simple and efficient maturation and specification of the desired cardiomyocyte subtype, facilitating both biomedical research and clinical applications.
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KEYWORD
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Cardiomyocyte, ascorbic acid, albumin, Wnt signaling
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